MIB1 Polyclonal Antibody

Rabbit Anti-MIB1 Polyclonal Antibody

Product Specifications

Background

MIB1 (mindbomb E3 ubiquitin protein ligase 1) encodes a protein containing multiple ankyrin repeats and RING finger domains that functions as an E3 ubiquitin ligase. The encoded protein positively regulates Notch signaling by ubiquitinating the Notch receptors, thereby facilitating their endocytosis. This protein may also promote the ubiquitination and degradation of death-associated protein kinase 1 (DAPK1) .

CAS Number

9007-83-4

Product Name Alternative

MIB1; DIP1; KIAA1323; ZZANK2

Gene ID

57534

Accession Number

Q86YT6

Reactivity

Human, Mouse, Rat

Immunogen

Synthetic peptide from human protein at AA range: 901-950

Clonality

Polyclonal

Tag

Mindbomb homolog 1 (Drosophila)

Applications

WB, IHC, IF, ELISA

Purification Method

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Molecular Weight

130kD

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Notes

The antibody detects endogenous MIB1 protein.

Observed Molecular Weight

130

Formulation

Liquid solution

Buffer pH

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Applications Notes

Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: IHC: 100-300; WB 1:500-2000; ELISA 1:10000-20000; IF 1:50-200