Tsg 101 Polyclonal Antibody

Rabbit Anti-Tsg 101 Polyclonal Antibody

Product Specifications

Background

The protein encoded by TSG101 (tumor susceptibility 101) belongs to a group of apparently inactive homologs of ubiquitin-conjugating enzymes. The gene product contains a coiled-coil domain that interacts with stathmin, a cytosolic phosphoprotein implicated in tumorigenesis. The protein may play a role in cell growth and differentiation and act as a negative growth regulator. In vitro steady-state expression of this tumor susceptibility gene appears to be important for maintenance of genomic stability and cell cycle regulation. Mutations and alternative splicing in TSG101 occur in high frequency in breast cancer and suggest that defects occur during breast cancer tumorigenesis and/or progression.

CAS Number

9007-83-4

Product Name Alternative

TSG101; Tumor susceptibility gene 101 protein; ESCRT-I complex subunit TSG101

Gene ID

7251

Accession Number

Q99816

Reactivity

Human, Mouse, Rat, Monkey

Immunogen

Synthesized peptide derived from the C-terminal region of human Tsg 101 at AA range: 250-330

Clonality

Polyclonal

Tag

Tumor susceptibility gene 101 protein

Applications

WB, IHC, IF, ELISA

Purification Method

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Molecular Weight

45kD

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Notes

Tsg 101 Polyclonal Antibody detects endogenous levels of Tsg 101 protein.

Observed Molecular Weight

Formulation

Liquid solution

Buffer pH

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Applications Notes

Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:5000; Not yet tested in other applications.