BUB1 Polyclonal Antibody

Rabbit Anti-BUB1 Polyclonal Antibody

Product Specifications

Background

BUB1 (BUB1 mitotic checkpoint serine/threonine kinase) encodes a serine/threonine-protein kinase that play a central role in mitosis. The encoded protein functions in part by phosphorylating members of the mitotic checkpoint complex and activating the spindle checkpoint. This protein also plays a role in inhibiting the activation of the anaphase promoting complex/cyclosome. This protein may also function in the DNA damage response. Mutations in BUB1 have been associated with aneuploidy and several forms of cancer. Alternate splicing results in multiple transcript variants.

CAS Number

9007-83-4

Product Name Alternative

BUB1; BUB1L; Mitotic checkpoint serine/threonine-protein kinase BUB1; hBUB1; BUB1A

Gene ID

699

Accession Number

O43683

Reactivity

Human, Mouse

Immunogen

Synthesized peptide derived from the Internal region of human BUB1 at AA range: 750-830

Clonality

Polyclonal

Tag

Mitotic checkpoint serine threonine-protein kinase BUB1

Applications

WB, ELISA

Purification Method

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Molecular Weight

120kD

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Notes

BUB1 Polyclonal Antibody detects endogenous levels of BUB1 protein.

Observed Molecular Weight

120

Formulation

Liquid solution

Buffer pH

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Applications Notes

Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000; ELISA 1:40000; Not yet tested in other applications.