SSB (phospho Ser366) Polyclonal Antibody

Rabbit Anti-SSB (phospho Ser366) Polyclonal Antibody

Product Specifications

Background

The protein encoded by SSB (Sjogren syndrome antigen B) is involved in diverse aspects of RNA metabolism, including binding and protecting poly (U) termini of nascent RNA polymerase III transcripts from exonuclease digestion, processing 5' and 3' ends of pre-tRNA precursors, acting as an RNA chaperone, and binding viral RNAs associated with hepatitis C virus. Autoantibodies reacting with this protein are found in the sera of patients with Sjogren syndrome and systemic lupus erythematosus. Alternative promoter usage results in two different transcript variants which encode the same protein.

CAS Number

9007-83-4

Product Name Alternative

SSB; Lupus La protein; La autoantigen; La ribonucleoprotein; Sjoegren syndrome type B antigen; SS-B

Gene ID

6741

Accession Number

P05455

Reactivity

Human, Rat, Mouse

Immunogen

Synthesized peptide derived from human SSB around the phosphorylation site of S366

Clonality

Polyclonal

Tag

Lupus La protein

Applications

WB, IHC, IF, ELISA

Purification Method

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Molecular Weight

47kD

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Notes

Phospho-SSB (S366) Polyclonal Antibody detects endogenous levels of SSB protein only when phosphorylated at S366.

Observed Molecular Weight

Formulation

Liquid solution

Buffer pH

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Applications Notes

Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:40000; Not yet tested in other applications.