Cleaved-Factor B Bb (K260) Polyclonal Antibody

CFB (complement factor B) encodes complement factor B, a component of the alternative pathway of complement activation. Factor B circulates in the blood as a single chain polypeptide. Upon activation of the alternative pathway, it is cleaved by complement factor D yielding the noncatalytic chain Ba and the catalytic subunit Bb. The active subunit Bb is a serine protease which associates with C3b to form the alternative pathway C3 convertase. Bb is involved in the proliferation of preactivated B lymphocytes, while Ba inhibits their proliferation. CFB localizes to the major histocompatibility complex (MHC) class III region on chromosome 6. This cluster includes several genes involved in regulation of the immune reaction. Polymorphisms in CFB are associated with a reduced risk of age-related macular degeneration. The polyadenylation site of CFB is 421 bp from the 5' end of the gene for complement component 2.

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Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Cleaved-Factor B Bb (K260) Polyclonal Antibody detects endogenous levels of fragment of activated Factor B Bb protein resulting from cleavage adjacent to K260.

Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000; ELISA 1:20000; Not yet tested in other applications.