HuB/D Polyclonal Antibody

Rabbit Anti-HuB/D Polyclonal Antibody

Product Specifications

Background

Eukaryotic translation initiation factor-5 (EIF5) interacts with the 40S initiation complex to promote hydrolysis of bound GTP with concomitant joining of the 60S ribosomal subunit to the 40S initiation complex. The resulting functional 80S ribosomal initiation complex is then active in peptidyl transfer and chain elongations (summary by Si et al. 1996 [PubMed 8663286]) .

CAS Number

9007-83-4

Product Name Alternative

ELAVL2; HUB; ELAV-like protein 2; ELAV-like neuronal protein 1; Hu-antigen B; HuB; Nervous system-specific RNA-binding protein Hel-N1; ELAVL4; HUD; PNEM; ELAV-like protein 4; Hu-antigen D; HuD; Paraneoplastic encephalomyelitis antigen HuD

Gene ID

1993/1996

Accession Number

Q12926/P26378

Reactivity

Human, Mouse, Rat

Immunogen

Synthesized peptide derived from the Internal region of human HuB/D at AA rangle: 30-110

Clonality

Polyclonal

Tag

ELAV-like protein 2

Applications

WB, IHC, IF, ELISA

Purification Method

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Molecular Weight

40kD

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Notes

HuB/D Polyclonal Antibody detects endogenous levels of HuB/D protein.

Observed Molecular Weight

Formulation

Liquid solution

Buffer pH

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Applications Notes

Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:40000; Not yet tested in other applications.