COL4A5 Polyclonal Antibody

Rabbit Anti-COL4A5 Polyclonal Antibody

Product Specifications

Background

COL4A5 encodes one of the six subunits of type IV collagen, the major structural component of basement membranes. Mutations in this gene are associated with X-linked Alport syndrome, also known as hereditary nephritis. Like the other members of the type IV collagen gene family, this gene is organized in a head-to-head conformation with another type IV collagen gene so that each gene pair shares a common promoter. Alternatively spliced transcript variants have been identified for this gene.

CAS Number

9007-83-4

Product Name Alternative

COL4A5; Collagen alpha-5 (IV) chain

Gene ID

1287

Accession Number

P29400

Reactivity

Human, Mouse

Immunogen

Synthesized peptide derived from the N-terminal region of human COL4A5 at AA rangle: 40-120

Clonality

Polyclonal

Tag

Collagen alpha-5 (IV) chain

Applications

IHC, IF, WB, ELISA

Purification Method

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Molecular Weight

160kD

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Notes

COL4A5 Polyclonal Antibody detects endogenous levels of COL4A5 protein.

Observed Molecular Weight

160

Formulation

Liquid solution

Buffer pH

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Applications Notes

Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:10000; Not yet tested in other applications.