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Rad52 Polyclonal Antibody

Rabbit Anti-Rad52 Polyclonal Antibody

Product Specifications

Background

RAD52 homolog, DNA repair protein encoded by RAD52 shares similarity with Saccharomyces cerevisiae Rad52, a protein important for DNA double-strand break repair and homologous recombination. RAD52 product was shown to bind single-stranded DNA ends, and mediate the DNA-DNA interaction necessary for the annealing of complementary DNA strands. It was also found to interact with DNA recombination protein RAD51, which suggested its role in RAD51 related DNA recombination and repair. A pseudogene of RAD52 is present on chromosome 2. Alternative splicing results in multiple transcript variants. Additional alternatively spliced transcript variants of RAD52 have been described, but their full-length nature is not known.

CAS Number

9007-83-4

Product Name Alternative

RAD52; DNA repair protein RAD52 homolog

Gene ID

5893

Accession Number

P43351

Reactivity

Human, Mouse

Immunogen

Synthesized peptide derived from human Rad52 around the non-phosphorylation site of Y104

Clonality

Polyclonal

Tag

DNA repair protein RAD52 homolog

Applications

WB, IHC, IF, ELISA

Purification Method

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Molecular Weight

46kD

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Notes

Rad52 Polyclonal Antibody detects endogenous levels of Rad52 protein.

Observed Molecular Weight

46

Formulation

Liquid solution

Buffer pH

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Applications Notes

Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:10000; Not yet tested in other applications.

Other Product Names

RAD52; DNA repair protein RAD52 homolog

Tested Applications

WB, IHC, IF, IP, ELISA

Host or Source

Rabbit

Accession Number URL

http://www.uniprot.org/uniprot/P43351

Isotype

Rabbit IgG

Available Sizes

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