PBFE Polyclonal Antibody

Rabbit Anti-PBFE Polyclonal Antibody

Product Specifications

Background

Enoyl-CoA hydratase and 3-hydroxyacyl CoA dehydrogenase by EHHADH is a bifunctional enzyme and is one of the four enzymes of the peroxisomal beta-oxidation pathway. The N-terminal region of the encoded protein contains enoyl-CoA hydratase activity while the C-terminal region contains 3-hydroxyacyl-CoA dehydrogenase activity. Defects in EHHADH are a cause of peroxisomal disorders such as Zellweger syndrome. Two transcript variants encoding different isoforms have been found for EHHADH.

CAS Number

9007-83-4

Product Name Alternative

EHHADH; ECHD; Peroxisomal bifunctional enzyme; PBE; PBFE

Gene ID

1962

Accession Number

Q08426

Reactivity

Human, Rat

Immunogen

Synthesized peptide derived from the Internal region of human PBFE at AA range: 450-530

Clonality

Polyclonal

Tag

Peroxisomal bifunctional enzyme

Applications

WB, IHC, IF, ELISA

Purification Method

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Molecular Weight

80kD

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Notes

PBFE Polyclonal Antibody detects endogenous levels of PBFE protein.

Observed Molecular Weight

Formulation

Liquid solution

Buffer pH

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Applications Notes

Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:10000; IF 1:50-200