DDX52 Polyclonal Antibody

Rabbit Anti-DDX52 Polyclonal Antibody

Product Specifications

Background

DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp, are putative RNA helicases implicated in several cellular processes involving modifications of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based on their distribution patterns, some members of this family may be involved in embryogenesis, spermatogenesis, and cellular growth and division. DEAD box protein 52 (DDX52), also known as ATP-dependent RNA helicase ROK1-like or HUSSY-19, is a 599 amino acid protein belonging to the DEAD box helicase family. Localized to the nucleus, DDX52 is phosphorylated by ATM or ATR upon DNA damage. DDX52 contains one helicase ATP-binding domain and one helicase C-terminal domain.

CAS Number

9007-83-4

Product Name Alternative

DDX52; ROK1; HUSSY-19; Probable ATP-dependent RNA helicase DDX52; ATP-dependent RNA helicase ROK1-like; DEAD box protein 52

Gene ID

11056

Accession Number

Q9Y2R4

Reactivity

Human, Rat, Mouse

Immunogen

Synthesized peptide derived from the C-terminal region of human DDX52 at AA range: 530-610

Clonality

Polyclonal

Tag

Probable ATP-dependent RNA helicase DDX52

Applications

WB, IHC, IF, ELISA

Purification Method

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Molecular Weight

67kD

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Notes

DDX52 Polyclonal Antibody detects endogenous levels of DDX52 protein.

Observed Molecular Weight

Formulation

Liquid solution

Buffer pH

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Applications Notes

Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:40000; IF 1:50-200