CdcA3 Polyclonal Antibody

Rabbit Anti-CdcA3 Polyclonal Antibody

Product Specifications

Background

Cell cycle events are regulated by the sequential activation and deactivation of cyclin dependent kinases (Cdks) and by the proteolysis of cyclins. The cell division control (Cdc) genes are required at various points in the cell cycle. CdcA3 (Cell division cycle-associated protein 3), also known as TOME1 (Trigger of mitotic entry protein 1) and GRCC8 (Gene-rich cluster protein C8), is a 268 amino acid cytoplasmic protein that is required for entry into mitosis. By participating with E3 ligase complexes, CdcA3 is involved in ubiquination and degradation of the mitosis-inhibitory kinase Wee 1, leading to the dephosphorylation of Cdc2 in the late G2 phase. CdcA3 itself is targeted for destruction by anaphase promoting complex (APC) in the G1 phase of the cell cycle, then allowing for Wee 1 accumulation during interphase.

CAS Number

9007-83-4

Product Name Alternative

CDCA3; C8; GRCC8; TOME1; Cell division cycle-associated protein 3; Gene-rich cluster protein C8; Trigger of mitotic entry protein 1; TOME-1

Gene ID

83461

Accession Number

Q99618

Reactivity

Human, Mouse, Rat

Immunogen

Synthesized peptide derived from the C-terminal region of human CdcA3 at AA range: 190-270

Clonality

Polyclonal

Tag

Cell division cycle-associated protein 3

Applications

WB, IHC, IF, ELISA

Purification Method

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Molecular Weight

28kD

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Notes

CdcA3 Polyclonal Antibody detects endogenous levels of CdcA3 protein.

Observed Molecular Weight

Formulation

Liquid solution

Buffer pH

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Applications Notes

Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:5000; Not yet tested in other applications.