Bub3 Polyclonal Antibody

Rabbit Anti-Bub3 Polyclonal Antibody

Product Specifications

Background

BUB3 encodes a protein involved in spindle checkpoint function. The encoded protein (BUB3, mitotic checkpoint protein) contains four WD repeat domains and has sequence similarity with the yeast BUB3 protein. Alternate transcriptional splice variants, encoding different isoforms, have been characterized.

CAS Number

9007-83-4

Product Name Alternative

BUB3; Mitotic checkpoint protein BUB3

Gene ID

9184

Accession Number

O43684

Reactivity

Human, Mouse, Rat

Immunogen

Synthesized peptide derived from the C-terminal region of human Bub3 at AA range: 260-340

Clonality

Polyclonal

Tag

Mitotic checkpoint protein BUB3

Applications

WB, IHC, IF, ELISA

Purification Method

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Molecular Weight

40kD

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Notes

Bub3 Polyclonal Antibody detects endogenous levels of Bub3 protein.

Observed Molecular Weight

Formulation

Liquid solution

Buffer pH

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Applications Notes

Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:5000; Not yet tested in other applications.