BCKDK Polyclonal Antibody

Rabbit Anti-BCKDK Polyclonal Antibody

Product Specifications

Background

The branched-chain alpha-ketoacid dehydrogenase complex (BCKD) is an important regulator of the valine, leucine, and isoleucine catabolic pathways. The protein (branched chain ketoacid dehydrogenase kinase) encoded by BCKDK is found in the mitochondrion, where it phosphorylates and inactivates BCKD. Several transcript variants encoding different isoforms have been found for BCKDK.

CAS Number

9007-83-4

Product Name Alternative

BCKDK; [3-methyl-2-oxobutanoate dehydrogenase [lipoamide]] kinase; mitochondrial; Branched-chain alpha-ketoacid dehydrogenase kinase; BCKD-kinase; BCKDHKIN

Gene ID

10295

Accession Number

O14874

Reactivity

Human, Mouse, Rat

Immunogen

Synthesized peptide derived from the N-terminal region of human BCKDK at AA range: 30-110

Clonality

Polyclonal

Tag

[3-methyl-2-oxobutanoate dehydrogenase [lipoamide]] kinase mitochondrial

Applications

WB, IHC, IF, ELISA

Purification Method

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Molecular Weight

46kD

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Notes

BCKDK Polyclonal Antibody detects endogenous levels of BCKDK protein.

Formulation

Liquid solution

Buffer pH

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Applications Notes

Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000; IHC 1:100-1:300; IF 1:200-1:1000; ELISA 1:20000; Not yet tested in other applications.

Other Product Names

BCKDK; [3-methyl-2-oxobutanoate dehydrogenase [lipoamide]] kinase; mitochondrial; Branched-chain alpha-ketoacid dehydrogenase kinase; BCKD-kinase; BCKDHKIN