PDGFR-β (phospho Tyr1021) Polyclonal Antibody

Rabbit Anti-PDGFR-β (phospho Tyr1021) Polyclonal Antibody

Product Specifications

Background

PDGFRB encodes a cell surface tyrosine kinase receptor (platelet derived growth factor receptor beta) for members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor alpha and beta polypeptides. This gene is flanked on chromosome 5 by the genes for granulocyte-macrophage colony-stimulating factor and macrophage-colony stimulating factor receptor; all three genes may be implicated in the 5-q syndrome. A translocation between chromosomes 5 and 12, that fuses PDGFRB to that of the translocation, ETV6, leukemia gene, results in chronic myeloproliferative disorder with eosinophilia.

CAS Number

9007-83-4

Product Name Alternative

PDGFRB; PDGFR; PDGFR1; Platelet-derived growth factor receptor beta; PDGF-R-beta; PDGFR-beta; Beta platelet-derived growth factor receptor; Beta-type platelet-derived growth factor receptor; CD140 antigen-like family member B; Platelet-deri

Gene ID

5159

Accession Number

P09619

Reactivity

Human, Mouse, Rat

Immunogen

Synthesized peptide derived from human PDGFR-β around the phosphorylation site of Y1021

Clonality

Polyclonal

Tag

Platelet-derived growth factor receptor beta

Applications

WB, IHC, IF, ELISA

Purification Method

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Molecular Weight

135-180kD

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Notes

Phospho-PDGFR-β (Y1021) Polyclonal Antibody detects endogenous levels of PDGFR-β protein only when phosphorylated at Y1021.

Formulation

Liquid solution

Buffer pH

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Applications Notes

Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-2000; IHC 1:100-1:300; ELISA 1:5000; IF 1:50-200