EEF2K (phospho Ser366) Polyclonal Antibody

Rabbit Anti-eEF2K (phospho Ser366) Polyclonal Antibody

Product Specifications

Background

EEF2K encodes a highly conserved protein kinase (eukaryotic elongation factor 2 kinase) in the calmodulin-mediated signaling pathway that links activation of cell surface receptors to cell division. This kinase is involved in the regulation of protein synthesis. It phosphorylates eukaryotic elongation factor 2 (EEF2) and thus inhibits the EEF2 function. The activity of eukaryotic elongation factor 2 kinase is increased in many cancers and may be a valid target for anti-cancer treatment.

CAS Number

9007-83-4

Product Name Alternative

EEF2K; Eukaryotic elongation factor 2 kinase; eEF-2 kinase; eEF-2K; Calcium/calmodulin-dependent eukaryotic elongation factor 2 kinase

Gene ID

29904

Accession Number

O00418

Reactivity

Human, Rat

Immunogen

Synthesized peptide derived from human eEF2K around the phosphorylation site of S366

Clonality

Polyclonal

Tag

Eukaryotic elongation factor 2 kinase

Applications

WB, IHC, IF, ELISA

Purification Method

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Molecular Weight

105kD

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Notes

Phospho-eEF2K (S366) Polyclonal Antibody detects endogenous levels of eEF2K protein only when phosphorylated at S366.

Observed Molecular Weight

105

Formulation

Liquid solution

Buffer pH

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Applications Notes

Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000; IHC 1:100-1:300; ELISA 1:40000; IF 1:50-200