Cortactin (Acetyl Lys235) Polyclonal Antibody

Rabbit Anti-Cortactin (Acetyl Lys235) Polyclonal Antibody

Product Specifications

Background

CTTN is overexpressed in breast cancer and squamous cell carcinomas of the head and neck. Cortactin is localized in the cytoplasm and in areas of the cell-substratum contacts. This gene has two roles: (1) regulating the interactions between components of adherens-type junctions and (2) organizing the cytoskeleton and cell adhesion structures of epithelia and carcinoma cells. During apoptosis, cortactin is degraded in a caspase-dependent manner. The aberrant regulation of CTTN contributes to tumor cell invasion and metastasis. Three splice variants that encode different isoforms have been identified for CTTN.

CAS Number

9007-83-4

Product Name Alternative

CTTN; EMS1; Src substrate cortactin; Amplaxin; Oncogene EMS1

Gene ID

2017

Accession Number

Q14247

Reactivity

Human, Mouse, Rat

Immunogen

Synthesized peptide derived from the Internal region of human Cortactin around the acetylation site of K235

Clonality

Polyclonal

Tag

Src substrate cortactin

Applications

WB, ELISA

Field of Research

Cancer#Signal Transduction

Purification Method

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Concentration

1 mg/ml

Molecular Weight

62kD

Precautions

The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

Shipping Conditions

Gel pack with blue ice.

Storage Conditions

Stable for one year at -20°C from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Aliquot to avoid repeated freezing and thawing.

Notes

Acetyl-Cortactin (K235) Polyclonal Antibody detects endogenous levels of Cortactin protein only when non-acetylation at K235.

Observed Molecular Weight

Formulation

Liquid solution

Buffer pH

Liquid in PBS containing 50% glycerol, 0.5% BSA and 0.02% sodium azide.

Applications Notes

Optimal working dilutions should be determined experimentally by the investigator. Suggested starting dilutions are as follows: WB 1:500-1:2000; ELISA 1:10000; Not yet tested in other applications.