TIGAR rabbit pAb

This gene is regulated as part of the p53 tumor suppressor pathway and encodes a protein with sequence similarity to the bisphosphate domain of the glycolytic enzyme that degrades fructose-2, 6-bisphosphate. The protein functions by blocking glycolysis and directing the pathway into the pentose phosphate shunt. Expression of this protein also protects cells from DNA damaging reactive oxygen species and provides some protection from DNA damage-induced apoptosis. The 12p13. 32 region that includes this gene is paralogous to the 11q13. 3 region. [provided by RefSeq, Jul 2008],

Product Specifications

Background

This gene is regulated as part of the p53 tumor suppressor pathway and encodes a protein with sequence similarity to the bisphosphate domain of the glycolytic enzyme that degrades fructose-2,6-bisphosphate. The protein functions by blocking glycolysis and directing the pathway into the pentose phosphate shunt. Expression of this protein also protects cells from DNA damaging reactive oxygen species and provides some protection from DNA damage-induced apoptosis. The 12p13.32 region that includes this gene is paralogous to the 11q13.3 region. [provided by RefSeq, Jul 2008]

UniProt

Q9NQ88

Swiss Prot

Q9NQ88

Reactivity

Human; Rat; Mouse

Immunogen

Synthesized peptide derived from part region of human protein

Target

TIGAR

Clonality

Polyclonal

Source

Rabbit

Applications

WB; ELISA

Concentration

1 mg/ml

Dilution

WB 1:500-2000 ELISA 1:5000-20000

Buffer

-20°C/1 year

Molecular Weight

29kD

Storage Conditions

-20°C/1 year

Observed Molecular Weight

29kD

Fragment

IgG

Subcellular Location

Cytoplasm . Nucleus . Mitochondrion . Translocated to the mitochondria during hypoxia in a HIF1A-dependent manner (PubMed:23185017) . Colocalizes with HK2 in the mitochondria during hypoxia (PubMed:23185017) . Translocated to the nucleus during hypoxia and/or genome stress-induced DNA damage responses in cancer cells (PubMed:25928429) . Translocation to the mitochondria is enhanced in ischemic cortex after reperfusion and/or during oxygen and glucose deprivation (OGD) /reoxygenation insult in primary neurons (By similarity) . .