CDC42 rabbit pAb

The protein encoded by this gene is a small GTPase of the Rho-subfamily, which regulates signaling pathways that control diverse cellular functions including cell morphology, migration, endocytosis and cell cycle progression. This protein is highly similar to Saccharomyces cerevisiae Cdc 42, and is able to complement the yeast cdc42-1 mutant. The product of oncogene Dbl was reported to specifically catalyze the dissociation of GDP from this protein. This protein could regulate actin polymerization through its direct binding to Neural Wiskott-Aldrich syndrome protein (N-WASP), which subsequently activates Arp2/3 complex. Alternative splicing of this gene results in multiple transcript variants. Pseudogenes of this gene have been identified on chromosomes 3, 4, 5, 7, 8 and 20. [provided by RefSeq, Apr 2013],

Product Specifications

Background

UniProt

P60953

Swiss Prot

P60953

Reactivity

Human; Mouse; Rat

Immunogen

Synthesized peptide derived from human protein . at AA range: 80-160

Target

CDC42

Clonality

Polyclonal

Source

Rabbit

Applications

WB; ELISA

Concentration

1 mg/ml

Dilution

WB 1:500-2000 ELISA 1:5000-20000

Buffer

-20°C/1 year

Molecular Weight

21kD

Storage Conditions

-20°C/1 year

Observed Molecular Weight

21kD

Fragment

IgG

Subcellular Location

Cell membrane ; Lipid-anchor ; Cytoplasmic side . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Cytoplasm, cytoskeleton, spindle . Midbody . Cell projection, dendrite . Localizes to spindle during prometaphase cells. Moves to the central spindle as cells progressed through anaphase to telophase (PubMed:15642749) . Localizes at the end of cytokinesis in the intercellular bridge formed between two daughter cells (PubMed:15642749) . Its localization is regulated by the activities of guanine nucleotide exchange factor ECT2 and GTPase activating protein RACGAP1 (PubMed:15642749) . Colocalizes with NEK6 in the centrosome (PubMed:20873783) . In its active GTP-bound form localizes to the leading edge membrane of migrating dendritic cells (By similarity) . .