RCC2 rabbit pAb

Function: Required for completion of mitosis and cytokinesis. May function as a guanine nucleotide exchange factor for the small GTPase RAC1. PTM: Phosphorylated upon DNA damage, probably by ATM or ATR. similarity: Contains 7 RCC1 repeats. subcellular location: Appears in the nucleus at G2, then concentrates at the inner centromere region of chromosomes during prophase. Redistributes to the midzone of the mitotic spindle during anaphase. Here, the protein covers the entire equatorial diameter from cortex to cortex. subunit: Binds preferentially to the nucleotide-free form of RAC1. Interacts with microtubules.

Product Specifications

Background

Function:Required for completion of mitosis and cytokinesis. May function as a guanine nucleotide exchange factor for the small GTPase RAC1., PTM:Phosphorylated upon DNA damage, probably by ATM or ATR., similarity:Contains 7 RCC1 repeats., subcellular location:Appears in the nucleus at G2, then concentrates at the inner centromere region of chromosomes during prophase. Redistributes to the midzone of the mitotic spindle during anaphase. Here, the protein covers the entire equatorial diameter from cortex to cortex., subunit:Binds preferentially to the nucleotide-free form of RAC1. Interacts with microtubules.

UniProt

Q9P258

Swiss Prot

Q9P258

Reactivity

Human; Mouse

Immunogen

The antiserum was produced against synthesized peptide derived from human RCC2. AA range:473-522

Clonality

Polyclonal

Source

Rabbit

Applications

WB; IHC; IF; ELISA

Concentration

1 mg/ml

Dilution

Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.

Molecular Weight

56kD

Storage Conditions

-20°C/1 year

Observed Molecular Weight

56kD

Fragment

IgG

Subcellular Location

Nucleus, nucleolus . Nucleus . Cytoplasm, cytoskeleton . Chromosome, centromere . Cytoplasm, cytoskeleton, spindle . Chromosome . Midbody . Cell membrane ; Peripheral membrane protein ; Cytoplasmic side . Appears in the nucleus at G2, then concentrates at the inner centromere region of chromosomes during prophase. Redistributes to the midzone of the mitotic spindle during anaphase. Here, the protein covers the entire equatorial diameter from cortex to cortex (PubMed:12919680, PubMed:1939370, PubMed:7559776, PubMed:9914378) . Colocalizes with cytoplasmic microtubules in interphase cells (PubMed:23388455) . Colocalizes with RAC1 at the cell membrane (PubMed:25074804) . .