HSF1 (phospho Ser121) rabbit pAb

Heat shock transcription factor 1 (HSF1) Homo sapiens The product of this gene is a transcription factor that is rapidly induced after temperature stress and binds heat shock promoter elements (HSE). This protein plays a role in the regulation of lifespan. Expression of this gene is repressed by phsphorylation, which promotes binding by heat shock protein 90. [provided by RefSeq, Aug 2016],

Product Specifications

Background

Heat shock transcription factor 1 (HSF1) Homo sapiens The product of this gene is a transcription factor that is rapidly induced after temperature stress and binds heat shock promoter elements (HSE) . This protein plays a role in the regulation of lifespan. Expression of this gene is repressed by phsphorylation, which promotes binding by heat shock protein 90. [provided by RefSeq, Aug 2016]

UniProt

Q00613

Swiss Prot

Q00613

Reactivity

Human; Mouse

Immunogen

The antiserum was produced against synthesized peptide derived from human HSF1 around the phosphorylation site of Ser121. AA range:87-136

Clonality

Polyclonal

Source

Rabbit

Applications

WB; IHC; IF; ELISA

Concentration

1 mg/ml

Dilution

Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.

Molecular Weight

50kD

Storage Conditions

-20°C/1 year

Observed Molecular Weight

50kD

Fragment

IgG

Subcellular Location

Nucleus . Cytoplasm . Nucleus, nucleoplasm . Cytoplasm, perinuclear region . Cytoplasm, cytoskeleton, spindle pole . Cytoplasm, cytoskeleton, microtubule organizing center, centrosome . Chromosome, centromere, kinetochore . The monomeric form is cytoplasmic in unstressed cells (PubMed:8455624, PubMed:26159920) . Predominantly nuclear protein in both unstressed and heat shocked cells (PubMed:10413683, PubMed:10359787) . Translocates in the nucleus upon heat shock (PubMed:8455624) . Nucleocytoplasmic shuttling protein (PubMed:26159920) . Colocalizes with IER5 in the nucleus (PubMed:27354066) . Colocalizes with BAG3 to the nucleus upon heat stress (PubMed:8455624, PubMed:26159920) . Localizes in subnuclear granules called nuclear stress bodies (nSBs) upon heat shock (PubMed:11447121, PubMed:1151455