Hec1 rabbit pAb

This gene encodes a component of the NDC80 kinetochore complex. The encoded protein consists of an N-terminal microtubule binding domain and a C-terminal coiled-coiled domain that interacts with other components of the complex. This protein functions to organize and stabilize microtubule-kinetochore interactions and is required for proper chromosome segregation. [provided by RefSeq, Oct 2011],

Product Specifications

Background

Product Name Alternative

NDC80; HEC; HEC1; KNTC2; Kinetochore protein NDC80 homolog; Highly expressed in cancer protein; Kinetochore protein Hec1; HsHec1; Kinetochore-associated protein 2; Retinoblastoma-associated protein HEC

UniProt

O14777

Swiss Prot

O14777

Reactivity

Human; Mouse

Immunogen

The antiserum was produced against synthesized peptide derived from human KNTC2. AA range:351-400

Target

Hec1

Clonality

Polyclonal

Source

Rabbit

Applications

WB; IF; ELISA

Concentration

1 mg/ml

Dilution

Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.

Buffer

-20°C/1 year

Molecular Weight

73kD

Storage Conditions

-20°C/1 year

Observed Molecular Weight

73kD

Fragment

IgG

Subcellular Location

Nucleus . Chromosome, centromere, kinetochore . Localizes to kinetochores from late prophase to anaphase (PubMed:14699129) . Localizes specifically to the outer plate of the kinetochore (PubMed:14699129) . .