Talin-1 rabbit pAb

This gene encodes a cytoskeletal protein that is concentrated in areas of cell-substratum and cell-cell contacts. The encoded protein plays a significant role in the assembly of actin filaments and in spreading and migration of various cell types, including fibroblasts and osteoclasts. It codistributes with integrins in the cell surface membrane in order to assist in the attachment of adherent cells to extracellular matrices and of lymphocytes to other cells. The N-terminus of this protein contains elements for localization to cell-extracellular matrix junctions. The C-terminus contains binding sites for proteins such as beta-1-integrin, actin, and vinculin. [provided by RefSeq, Feb 2009],

Product Specifications

Background

Product Name Alternative

TLN1; KIAA1027; TLN; Talin-1

UniProt

Q9Y490

Swiss Prot

Q9Y490

Reactivity

Human; Mouse; Rat

Immunogen

The antiserum was produced against synthesized peptide derived from the C-terminal region of human TLN1. AA range:2471-2520

Target

Talin

Clonality

Polyclonal

Source

Rabbit

Applications

WB; IHC; IF; ELISA

Concentration

1 mg/ml

Dilution

Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. IF 1:100-300 Not yet tested in other applications.

Buffer

-20°C/1 year

Molecular Weight

269kD

Storage Conditions

-20°C/1 year

Observed Molecular Weight

269kD

Fragment

IgG

Subcellular Location

Cell projection, ruffle membrane ; Peripheral membrane protein ; Cytoplasmic side . Cytoplasm, cytoskeleton . Cell surface . Cell junction, focal adhesion . Colocalizes with LAYN at the membrane ruffles. Localized preferentially in focal adhesions than fibrillar adhesions (By similarity) . .