FGF-2 rabbit pAb

The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. This protein has been implicated in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. The mRNA for this gene contains multiple polyadenylation sites, and is alternatively translated from non-AUG (CUG) and AUG initiation codons, resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF. [provided by RefSeq, Jul 2008],

Product Specifications

Background

Product Name Alternative

FGF2; FGFB; Fibroblast growth factor 2; FGF-2; Basic fibroblast growth factor; bFGF; Heparin-binding growth factor 2; HBGF-2

UniProt

P09038

Swiss Prot

P09038

Reactivity

Human; Mouse; Rat

Immunogen

The antiserum was produced against synthesized peptide derived from the Internal region of human FGF2. AA range:151-200

Target

FGF-2

Clonality

Polyclonal

Source

Rabbit

Applications

WB; IHC; IF; ELISA

Concentration

1 mg/ml

Dilution

Western Blot: 1/500 - 1/2000. IHC-p: 1:100-1:300. ELISA: 1/10000. Not yet tested in other applications.

Buffer

-20°C/1 year

Molecular Weight

30kD

Storage Conditions

-20°C/1 year

Observed Molecular Weight

30kD

Fragment

IgG

Subcellular Location

Secreted . Nucleus . Exported from cells by an endoplasmic reticulum (ER) /Golgi-independent mechanism. Unconventional secretion of FGF2 occurs by direct translocation across the plasma membrane (PubMed:20230531) . Binding of exogenous FGF2 to FGFR facilitates endocytosis followed by translocation of FGF2 across endosomal membrane into the cytosol (PubMed:22321063) . Nuclear import from the cytosol requires the classical nuclear import machinery, involving proteins KPNA1 and KPNB1, as well as CEP57 (PubMed:22321063) . .