Rab 5A rabbit pAb

Enzyme regulation: Regulated by guanine nucleotide exchange factors (GEFs) which promote the exchange of bound GDP for free GTP. function: Required for the fusion of plasma membranes and early endosomes. similarity: Belongs to the small GTPase superfamily. Rab family. subcellular location: Enriched in stage I melanosomes. subunit: Binds EEA1. Interacts with RIN1 and GAPVD1, which regulate its pathway, probably by acting as a GEF. Interacts with ALS2CL, UNC84B, ZFYVE20 and RUFY1. Interacts with SGSM1 and SGSM3.

Product Specifications

Background

Enzyme regulation:Regulated by guanine nucleotide exchange factors (GEFs) which promote the exchange of bound GDP for free GTP., function:Required for the fusion of plasma membranes and early endosomes., similarity:Belongs to the small GTPase superfamily. Rab family., subcellular location:Enriched in stage I melanosomes., subunit:Binds EEA1. Interacts with RIN1 and GAPVD1, which regulate its pathway, probably by acting as a GEF. Interacts with ALS2CL, UNC84B, ZFYVE20 and RUFY1. Interacts with SGSM1 and SGSM3.

Product Name Alternative

RAB5A; RAB5; Ras-related protein Rab-5A

UniProt

P20339

Swiss Prot

P20339

Reactivity

Human; Mouse; Rat

Immunogen

The antiserum was produced against synthesized peptide derived from the Internal region of human RAB5A. AA range:71-120

Target

Rab 5A

Clonality

Polyclonal

Source

Rabbit

Applications

WB; IHC; IF; ELISA

Concentration

1 mg/ml

Dilution

Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.

Buffer

-20°C/1 year

Molecular Weight

24kD

Storage Conditions

-20°C/1 year

Observed Molecular Weight

24kD

Fragment

IgG

Subcellular Location

Cell membrane ; Lipid-anchor ; Cytoplasmic side . Early endosome membrane ; Lipid-anchor . Melanosome . Cytoplasmic vesicle . Cell projection, ruffle . Membrane . Cytoplasm, cytosol . Cytoplasmic vesicle, phagosome membrane . Endosome membrane . Enriched in stage I melanosomes (PubMed:17081065) . Alternates between membrane-bound and cytosolic forms (Probable) . .