SLAM rabbit pAb

Domain: The most membrane-proximal SH2-binding motif interacts with SH2 domain of SH2D1A and does not need to be phosphorylated on tyrosine residues. function: High-affinity self-ligand important in bidirectional T-cell to B-cell stimulation. SLAM-induced signal-transduction events in T-lymphocytes are different from those in B-cells. Two modes of SLAM signaling are likely to exist: one in which the inhibitor SH2D1A acts as a negative regulator and another in which protein-tyrosine phosphatase 2C (PTPN11)-dependent signal transduction operates. PTM: Phosphorylated by FYN. similarity: Contains 1 Ig-like C2-type (immunoglobulin-like) domain. similarity: Contains 1 Ig-like V-type (immunoglobulin-like) domain. subcellular location: Present on the surface of B-cells and T-cells. subunit: Its cytoplasmic domain interacts with SH2 domain protein 1A (SH2D1A), and with PTPN11. Interacts with INPP5D/SHIP1. Binds to Measles virus HN protein and acts as a receptor for this virus. tissue specificity: Constitutively expressed on peripheral blood memory T-cells, T-cell clones, immature thymocytes, and a proportion of B-cells, and is rapidely induced on naive T-cells after activation.

Product Specifications

Background

Domain:The most membrane-proximal SH2-binding motif interacts with SH2 domain of SH2D1A and does not need to be phosphorylated on tyrosine residues., function:High-affinity self-ligand important in bidirectional T-cell to B-cell stimulation. SLAM-induced signal-transduction events in T-lymphocytes are different from those in B-cells. Two modes of SLAM signaling are likely to exist: one in which the inhibitor SH2D1A acts as a negative regulator and another in which protein-tyrosine phosphatase 2C (PTPN11) -dependent signal transduction operates., PTM:Phosphorylated by FYN., similarity:Contains 1 Ig-like C2-type (immunoglobulin-like) domain., similarity:Contains 1 Ig-like V-type (immunoglobulin-like) domain., subcellular location:Present on the surface of B-cells and T-cells., subunit:Its cytoplasmic domain interacts with SH2 domain protein 1A (SH2D1A), and with PTPN11. Interacts with INPP5D/SHIP1. Binds to Measles virus HN protein and acts as a receptor for this virus., tissue specificity:Constitutively expressed on peripheral blood memory T-cells, T-cell clones, immature thymocytes, and a proportion of B-cells, and is rapidely induced on naive T-cells after activation.

Product Name Alternative

SLAMF1; SLAM; Signaling lymphocytic activation molecule; CDw150; IPO-3; CD150

UniProt

Q13291

Swiss Prot

Q13291

Reactivity

Human; Rat; Mouse

Immunogen

The antiserum was produced against synthesized peptide derived from the Internal region of human SLAMF1. AA range:81-130

Target

SLAM

Clonality

Polyclonal

Source

Rabbit

Applications

WB; IHC; IF; ELISA

Concentration

1 mg/ml

Dilution

Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.

Buffer

-20°C/1 year

Molecular Weight

37kD

Storage Conditions

-20°C/1 year

Observed Molecular Weight

37kD

Fragment

IgG

Subcellular Location

Cell membrane ; Single-pass type I membrane protein. Present on the surface of B-cells and T-cells. Located at the plasma membrane contacts between neighboring T-cells (PubMed:11806999) . .; [Isoform 3]: Secreted .; [Isoform 4]: Cell membrane . Overexpressed isoform 4 is detected on the cell surface. In glioma cell lines endogenuous isoform 4 is detetced predominantly in the cytoplasm and colocalized with endoplasmic reticulum and Golgi markers. .