Septin 7 rabbit pAb

Septin 7 (SEPT7) Homo sapiens This gene encodes a protein that is highly similar to the CDC10 protein of Saccharomyces cerevisiae. The protein also shares similarity with Diff 6 of Drosophila and with H5 of mouse. Each of these similar proteins, including the yeast CDC10, contains a GTP-binding motif. The yeast CDC10 protein is a structural component of the 10 nm filament which lies inside the cytoplasmic membrane and is essential for cytokinesis. This human protein functions in gliomagenesis and in the suppression of glioma cell growth, and it is required for the association of centromere-associated protein E with the kinetochore. Alternative splicing results in multiple transcript variants. Several related pseudogenes have been identified on chromosomes 5, 7, 9, 10, 11, 14, 17 and 19. [provided by RefSeq, Jul 2011],

Product Specifications

Background

UniProt

Q16181

Swiss Prot

Q16181

Reactivity

Human; Mouse; Rat

Immunogen

The antiserum was produced against synthesized peptide derived from human SEPT7. AA range:350-399

Clonality

Polyclonal

Source

Rabbit

Applications

WB; IHC; IF; ELISA

Concentration

1 mg/ml

Dilution

Western Blot: 1/500 - 1/2000.IHC-p:1:50-300 ELISA: 1/20000. Not yet tested in other applications.

Molecular Weight

48kD

Storage Conditions

-20°C/1 year

Observed Molecular Weight

48kD

Fragment

IgG

Subcellular Location

Cytoplasm . Chromosome, centromere, kinetochore . Cytoplasm, cytoskeleton, spindle . Cleavage furrow . Midbody . Cytoplasm, cytoskeleton, cilium axoneme . Cell projection, cilium, flagellum . Distributed throughout the cytoplasm in prometaphase cells. Associated with the spindle during metaphase. Associated with the central spindle and at the cleavage furrow in anaphase cells. Detected at the midbody in telophase. Associated with actin stress fibers (By similarity) . Found in the sperm annulus (PubMed:25588830) . .