P53RFP rabbit pAb

Caution: Lacks the His residue in the RING-type domain 2 that is one of the conserved features of the family. Domain: The RING-type zinc finger domain mediates binding to an E2 ubiquitin-conjugating enzyme. function: E3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating enzymes UBE2L3 and UBE2L6 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates such as LCMT2, thereby promoting their degradation. Induces apoptosis via a TP53/p53-dependent but caspase-independent mechanism. pathway: Protein modification; protein ubiquitination. PTM: Auto-ubiquitinated. similarity: Belongs to the RBR family. RNF144 subfamily. similarity: Contains 1 IBR-type zinc finger. similarity: Contains 2 RING-type zinc fingers. subunit: Interacts with UBE2L3, UBE2L6 and LCMT2. tissue specificity: Broadly expressed, with lowest levels in brain, spleen and thymus.

Product Specifications

Background

Caution:Lacks the His residue in the RING-type domain 2 that is one of the conserved features of the family., domain:The RING-type zinc finger domain mediates binding to an E2 ubiquitin-conjugating enzyme., function:E3 ubiquitin-protein ligase which accepts ubiquitin from E2 ubiquitin-conjugating enzymes UBE2L3 and UBE2L6 in the form of a thioester and then directly transfers the ubiquitin to targeted substrates such as LCMT2, thereby promoting their degradation. Induces apoptosis via a TP53/p53-dependent but caspase-independent mechanism., pathway:Protein modification; protein ubiquitination., PTM:Auto-ubiquitinated., similarity:Belongs to the RBR family. RNF144 subfamily., similarity:Contains 1 IBR-type zinc finger., similarity:Contains 2 RING-type zinc fingers., subunit:Interacts with UBE2L3, UBE2L6 and LCMT2., tissue specificity:Broadly expressed, with lowest levels in brain, spleen and thymus.

Product Name Alternative

RNF144B; IBRDC2; P53RFP; E3 ubiquitin-protein ligase RNF144B; IBR domain-containing protein 2; RING finger protein 144B; p53-inducible RING finger protein

UniProt

Q7Z419

Swiss Prot

Q7Z419

Reactivity

Human; Mouse

Immunogen

Synthesized peptide derived from the Internal region of human p53RFP.

Target

P53RFP

Clonality

Polyclonal

Source

Rabbit

Applications

WB; ELISA

Concentration

1 mg/ml

Dilution

Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.

Buffer

-20°C/1 year

Molecular Weight

35kD

Storage Conditions

-20°C/1 year

Observed Molecular Weight

35kD

Fragment

IgG

Subcellular Location

Mitochondrion membrane ; Single-pass membrane protein . Cytoplasm . Mostly cytosololic, accumulates in submitochondrial domains specifically upon apoptosis induction, in synchrony with BAX activation.

Other Product Names

RNF144B; IBRDC2; P53RFP; E3 ubiquitin-protein ligase RNF144B; IBR domain-containing protein 2; RING finger protein 144B; p53-inducible RING finger protein