MRCKβ rabbit pAb

This gene encodes a member of the serine/threonine protein kinase family. The encoded protein contains a Cdc42/Rac-binding p21 binding domain resembling that of PAK kinase. The kinase domain of this protein is most closely related to that of myotonic dystrophy kinase-related ROK. Studies of the similar gene in rat suggested that this kinase may act as a downstream effector of Cdc42 in cytoskeletal reorganization. [provided by RefSeq, Jul 2008],

Product Specifications

Background

UniProt

Q9Y5S2

Swiss Prot

Q9Y5S2

Reactivity

Human; Mouse; Rat

Immunogen

The antiserum was produced against synthesized peptide derived from human MRCKB. AA range:1641-1690

Clonality

Polyclonal

Source

Rabbit

Applications

WB; IHC; IF; ELISA

Concentration

1 mg/ml

Dilution

Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/40000. Not yet tested in other applications.

Molecular Weight

194kD

Storage Conditions

-20°C/1 year

Observed Molecular Weight

194kD

Fragment

IgG

Subcellular Location

Cytoplasm . Cell membrane ; Peripheral membrane protein ; Cytoplasmic side . Cell junction . Cell projection, lamellipodium . Displays a dispersed punctate distribution and concentrates along the cell periphery, especially at the leading edge and cell-cell junction. This concentration is PH-domain dependent (By similarity) . Detected at the leading edge of migrating cells. Localization at the leading edge of migrating cells requires interaction with catalytically active CDC42 (PubMed:21240187) . Localizes in the lamellipodium in a FAM89B/LRAP25-dependent manner (By similarity) . .

Other Product Names

CDC42BPB; KIAA1124; Serine/threonine-protein kinase MRCK beta; CDC42-binding protein kinase beta; CDC42BP-beta; DMPK-like beta; Myotonic dystrophy kinase-related CDC42-binding kinase beta; MRCK beta; Myotonic dystrophy protein kinase-like b