DGK-ι rabbit pAb

This gene is a member of the type IV diacylglycerol kinase subfamily. Diacylglycerol kinases regulate the intracellular concentration of diacylglycerol through its phosphorylation, producing phosphatidic acid. The specific role of the enzyme encoded by this gene is undetermined, however, it may play a crucial role in the production of phosphatidic acid in the retina or in recessive forms of retinal degeneration. [provided by RefSeq, Jul 2008],

Product Specifications

Background

Product Name Alternative

DGKI; Diacylglycerol kinase iota; DAG kinase iota; Diglyceride kinase iota; DGK-iota

UniProt

O75912

Swiss Prot

O75912

Reactivity

Human; Rat

Immunogen

The antiserum was produced against synthesized peptide derived from human DGKI. AA range:991-1040

Target

DGK-ι

Clonality

Polyclonal

Source

Rabbit

Applications

WB; IHC; IF; ELISA

Concentration

1 mg/ml

Dilution

Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.

Buffer

-20°C/1 year

Molecular Weight

160kD

Storage Conditions

-20°C/1 year

Observed Molecular Weight

160kD

Fragment

IgG

Subcellular Location

Cell projection, axon . Cell projection, dendrite . Cell junction, synapse, presynapse . Cell junction, synapse, postsynapse . Cell junction, synapse, postsynaptic density . Cell junction, synapse, synaptic cell membrane . Cytoplasmic vesicle, secretory vesicle, synaptic vesicle membrane . Cytoplasm, cytosol . Nucleus . Excluded from inhibitory synapses (By similarity) . Localization between cytoplasm and nucleus is regulated by protein kinase C (PubMed:9830018) . Both in the detergent soluble and particulate fractions (By similarity) . .