DGK-δ rabbit pAb

This gene encodes a cytoplasmic enzyme that phosphorylates diacylglycerol to produce phosphatidic acid. Diacylglycerol and phosphatidic acid are two lipids that act as second messengers in signaling cascades. Their cellular concentrations are regulated by the encoded protein, and so it is thought to play an important role in cellular signal transduction. Alternative splicing results in two transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008],

Product Specifications

Background

UniProt

Q16760

Swiss Prot

Q16760

Reactivity

Human; Rat; Mouse

Immunogen

The antiserum was produced against synthesized peptide derived from human DGKD. AA range:41-90

Clonality

Polyclonal

Source

Rabbit

Applications

WB; IHC; IF; ELISA

Concentration

1 mg/ml

Dilution

Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.

Molecular Weight

135kD

Storage Conditions

-20°C/1 year

Observed Molecular Weight

135kD

Fragment

IgG

Subcellular Location

Membrane, clathrin-coated pit . Cytoplasm .; [Isoform 1]: Cell membrane ; Peripheral membrane protein . Cytoplasm . Isoform 1 translocation from cytoplasm to the plasma membrane is induced by phorbol esters (PubMed:12200442) . Phorbol esters induce the conversion into the monomeric form which can translocate to the plasma membrane (PubMed:12084710) . .