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GAPDH Mouse mAb

Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. GAPDH is constitutively expressed in almost all tissues at high levels, therefore antibodies against GAPDH are useful as loading controls for Western Blotting. Some physiological factors, such as hypoxia and diabetes, increase GAPDH expression in certain cell types.

Product Specifications

Background

Glyceraldehyde 3 phosphate dehydrogenase (GAPDH) is well known as one of the key enzymes involved in glycolysis. GAPDH is constitutively expressed in almost all tissues at high levels, therefore antibodies against GAPDH are useful as loading controls for Western Blotting. Some physiological factors, such as hypoxia and diabetes, increase GAPDH expression in certain cell types.

UniProt

P04406

Swiss Prot

P04406

Reactivity

Human, Rat, Mouse, Monkey, Dog, Chicken, Hamster, Rabbit, Pig, Sheep, Insect, Yeast

Immunogen

Synthetic Peptide

Clonality

Monoclonal

Source

Mouse

Applications

WB, IHC, IF

Concentration

1mg/mL

Dilution

WB 1:5,000 IHC 1:200

Buffer

PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.

Molecular Weight

37kDa

Storage Conditions

PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.

Observed Molecular Weight

37kDa

Fragment

IgG1

Subcellular Location

Cytoplasm, Cytoskeleton, Membrane, Nucleus

Gene ID (Human)

2597

Available Sizes

Curated Selection

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