Flag-Tag (HRP Conjugated) Mouse mAb
The DYKDDDDK peptide (Flag-tag) is a small component of an epitope which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. It has been used extensively as a general epitope tag in expression vectors. It can be used for affinity chromatography, then used to separate recombinant, overexpressed protein from wild-type protein expressed by the host organism. It can also be used in the isolation of protein complexes with multiple subunits. A Flag-tag can be used in many different assays that require recognition by an antibody. If there is no antibody against the studied protein, adding a Flag-tag to this protein allows one to follow the protein with an antibody against the Flag sequence.
Product Specifications
Background
Synonyms
DDDDK epitope tag, DYKDDDDK epitope tag, ECS epitope tag
Host
Mouse
Immunogen
Synthetic Peptide
Clonality
Monoclonal
Isotype
IgG1
Source
Mouse
Applications
WB
Validated Applications
WB
Stability
PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.
Concentration
1 mg/mL
Dilution
WB 1:5,000-10,000
Storage Conditions
PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.
Product Datasheet
https://www.elkbiotech.com/upload/file/Antibodies/mAb/EM1024-1.pdf
Other Product Names
DDDDK epitope tag, DYKDDDDK epitope tag, ECS epitope tag
Available Sizes
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