Proteinase K

Proteinase K (Protease K) is a nonspecific serine protease that is useful for general digestion of proteins. Proteinase K is active in the presence of SDS or urea and over a wide range of pH (4-12), salt concentrations, and temperatures. Proteinase K can be use for promoting methods of viral nucleic acid extraction, and detection[1][2][3][4].

Product Specifications

CAS Number

[39450-01-6]

Product Name Alternative

Protease K

UNSPSC

12352211

Hazard Statement

H334

Target

Ser/Thr Protease

Type

Reference compound

Related Pathways

Metabolic Enzyme/Protease

Field of Research

Others

Assay Protocol

https://www.medchemexpress.com/protease-k.html

Solubility

H2O : 25 mg/mL (ultrasonic)

Smiles

[ProteinaseK]

Precautions

H334

References & Citations

[1]W Ebeling, et al. Proteinase K From Tritirachium Album Limber. Eur J Biochem . 1974 Aug 15;47 (1) :91-7.|[2]Nosrati H, et al. Multifunctional nanoparticles from albumin for stimuli-responsive efficient dual drug delivery. Bioorg Chem. 2019 Jul;88:102959. |[3]Shukla A, et al. Vitality of Proteinase K in rRTPCR Detection of SARS-CoV2 Bypassing RNA Extraction. Front Cell Infect Microbiol. 2021 Nov 3;11:717068. |[4]Yu F, et al. Comparative Evaluation of Three Preprocessing Methods for Extraction and Detection of Influenza A Virus Nucleic Acids from Sputum. Front Med (Lausanne) . 2018 Mar 2;5:56.