AF488 NHS ester

AF488 NHS ester is an amine specific fluorescence probe (Em=525 nm) . AF488 NHS ester reacts with sulfhydryl groups and amines in aqueous and biological samples then change their chemical structure and fluorescence properties after derivatization[1].

Product Specifications

CAS Number

[1374019-99-4]

UNSPSC

12171500

Hazard Statement

H302, H315, H319

Target

Fluorescent Dye

Type

Dye Reagents

Related Pathways

Others

Field of Research

Others

Assay Protocol

https://www.medchemexpress.com/af488-nhs-ester.html

Purity

95.50

Solubility

10 mM in DMSO

Smiles

O=C(C1=C(C2=C(C=CC(N)=C3S(=O)(O)=O)C3=[O+]C4=C2C=CC(N)=C4S(=O)([O-])=O)C=CC(C(ON5C(CCC5=O)=O)=O)=C1)O

Molecular Formula

C25H17N3O13S2

Molecular Weight

631.54

Precautions

H302, H315, H319

References & Citations

[1]Kendall CG, et al. Amine Analysis Using AlexaFluor 488 Succinimidyl Ester and Capillary Electrophoresis with Laser-Induced Fluorescence. J Anal Methods Chem. 2015;2015:368362.|[2]Lavoie RA, et al. Targeted Capture of Chinese Hamster Ovary Host Cell Proteins: Peptide Ligand Discovery. Int J Mol Sci. 2019 Apr 8;20 (7) :1729.