Anti-IKKγ (NT) Antibody

Rabbit Polyclonal Antibody specific to IKK γ (NT)

Product Specifications

CAS Number

9007-83-4

Product Name Alternative

NEMO, FIP-3, IkB kinase-associated protein 1, IKKAP1, Inhibitor of nuclear factor kappa-B kinase subunit γ, I-kappa-B kinase subunit γ, IKK-γ, IKKG, IkB kinase subunit γ, NF-kappa-B essential modifier

Gene Name

IKBKG

Gene ID

8517

NCBI Gene ID

<a href="https://www.ncbi.nlm.nih.gov/gene/?term=IKBKG">IKBKG</a>

UniProt

Q9Y6K9

Accession Number

NP_001093326

Cellular Locus

Cytoplasm, Nucleus

Host

Rabbit

Reactivity

Human, Mouse, Rat

Immunogen

Peptide corresponding to aa 400-416 of human IKKg. This sequence is identical to that of mouse.

Target Antigen

NF-kappa-B essential modulator

Target

IKKγ (NT)

Clonality

Polyclonal

Isotype

IgG

Type

Antibody

Applications

ELISA, WB, ICC, IF

Field of Research

Neuroscience

Purification Method

Purified by peptide immuno-affinity chromatography

Concentration

Lot Specific

Dilution

Dilute in PBS or medium which is identical to that used in the assay system.

Format

Purified

Form

Liquid

Buffer

Phosphate Buffered Saline

Function

Regulatory subunit of the IKK core complex which phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor (PubMed:9751060, PubMed:14695475, PubMed:20724660). Its binding to scaffolding polyubiquitin plays a key role in IKK activation by multiple signaling receptor pathways (PubMed:16547522, PubMed:18287044, PubMed:19033441, PubMed:21606507, PubMed:27777308, PubMed:19185524, PubMed:33567255). Can rPubMed:14695475, PubMed:16547522, PubMed:18287044, PubMed:19033441, PubMed:19185524, PubMed:19854139, PubMed:20724660, PubMed:21606507, PubMed:27777308, PubMed:33567255, PubMed:9751060}.; (Microbial infection) Also considered to be a mediator for HTLV-1 Tax oncoprotein activation of NF-kappa-B. {PubMed:10364167, PubMed:11064457}.

Additionnal Information

Immunoblotting: use at 1:500-1:1,000 dilution. <br><br>Positive control: Whole cell lysate from HeLa cells.

Storage Conditions

This antibody is stable for at least one (1) year at -20°C. Avoid multiple freeze- thaw cycles.

Specificity

This antibody recognizes human, mouse, and rat IKKg (52 kD). No cross-reactivity with IKKalpha, IKKb or IKKepsilon.

Formulation

PBS, pH 7.4.

Buffer pH

pH 7.4

Target Background

Nuclear factor kappa B (NF-kappaB) is a ubiquitous transcription factor and key mediator of gene expression during immune and inflammatory responses. NF-kappaB activates numerous genes in response to extracellular stimuli, such as IL-1, TNFalpha, and LPS. NF-kappaB is associated with IkappaB in cytoplasm, which inhibits NF-kappaB activity. IkappaB is phos-phorylated by the IkappaB kinase (IKK) complex which contains IKKalpha, IKKbeta, and IKKepsilon. A novel molecule in the IKK complex, designated IKKg, interacts with IKKbeta and is required for the activation of IKK complex and NF-kappaB by LPS, PMA, TNF, and IL-1. IKKg also binds to RIP and NIK and mediates TNF-induced NF-kappaB activation.