Biotinyl tyramide

Biotinyl tyramide is a biotin derivative used for tyramide signal amplification (TSA), as a reagent to amplify both immunohistochemical signals and in situ hybridization protocols. Biotinyl tyramide can be used for the research of tyramide signal amplification[1][2][3][4][5].

Product Specifications

CAS Number

[41994-02-9]

UNSPSC

12171500

Hazard Statement

H302, H315, H319, H335

Target

Fluorescent Dye

Type

Dye Reagents

Related Pathways

Others

Field of Research

Others

Assay Protocol

https://www.medchemexpress.com/biotinyl-tyramide.html

Concentration

10mM

Purity

99.97

Solubility

DMSO : 100 mg/mL (ultrasonic)

Smiles

O=C(NCCC1=CC=C(O)C=C1)CCCC[C@@H]2SC[C@]([C@]2([H])N3)([H])NC3=O

Molecular Formula

C18H25N3O3S

Molecular Weight

363.47

Precautions

H302, H315, H319, H335

References & Citations

[1]Bobrow M N, et al. Catalyzed reporter deposition, a novel method of signal amplification application to immunoassays [J]. Journal of immunological methods, 1989, 125 (1-2) : 279-285.|[2]Kim S H, et al. An improved protocol of biotinylated tyramine-based immunohistochemistry minimizing nonspecific background staining [J]. Journal of Histochemistry & Cytochemistry, 2003, 51 (1) : 129-132. |[3]Hunyady B, et al. Immunohistochemical signal amplification by catalyzed reporter deposition and its application in double immunostaining [J]. Journal of Histochemistry & Cytochemistry, 1996, 44 (12) : 1353-1362.|[4]Evans M F, et al. Optimization of biotinyl-tyramide-based in situ hybridization for sensitive background-free applications on formalin-fixed, paraffin-embedded tissue specimens [J]. BMC Clinical Pathology, 2003, 3: 1-17. |[5]Kharel P, et al. Stress promotes RNA G-quadruplex folding in human cells [J]. Nature Communications, 2023, 14 (1) : 205.|[6]Pató A, et al. Hydrogen peroxide production by epidermal dual oxidase 1 regulates nociceptive sensory signals [J]. Redox Biology, 2023, 62: 102670.|[7]Dráberová E, et al. Quantification of α-tubulin isotypes by sandwich ELISA with signal amplification through biotinyl-tyramide or immuno-PCR [J]. Journal of immunological methods, 2013, 395 (1-2) : 63-70.