X-Gluc (sodium)

X-Gluc sodium is a dye reagent for the detection of β-glucuronidase (GUS), an enzyme produced by E. coli. X-Gluc sodium can be used to detect E. coli contamination in food, water and the urinary tract (GUS as a specific detection indicator) . X-Gluc sodium is also widely used in molecular biology experiments to label and detect the expression of target genes (reacts with the GUS gene, appears blue) [1].

Product Specifications

CAS Number

[129541-41-9]

UNSPSC

12171500

Hazard Statement

H302, H315, H319, H335

Target

Fluorescent Dye; β-glucuronidase

Type

Dye Reagents

Related Pathways

Metabolic Enzyme/Protease; Others

Field of Research

Others

Assay Protocol

https://www.medchemexpress.com/x-gluc-sodium.html

Purity

98.0

Solubility

DMSO : ≥ 100 mg/mL

Smiles

O[C@H]([C@H]([C@@H]([C@@H](C(O[Na])=O)O1)O)O)[C@@H]1OC2=CNC3=C2C(Cl)=C(Br)C=C3

Molecular Formula

C14H12BrClNNaO7

Molecular Weight

444.59

Precautions

H302, H315, H319, H335

References & Citations

[1]Elon W Frampton, et al. Evaluation of the β-Glucuronidase Substrate 5-Bromo-4-Chloro-3-Indolyl-β-D-Glucuronide (X-GLUC) in a 24-Hour Direct Plating Method for Escherichia coli. J Food Prot. 1988 May;51 (5) :402-404.