Phalloidin:FITC

Product Specifications

Background

Phalloidin is a fungal toxin isolated from the poisonous mushroom Amanita phalloides. Its toxicity is attributed to the ability to bind F-actin in liver and muscle cells. As a result of binding phalloidin, actin filaments become strongly stabilized. Phalloidin has been found to bind only to polymeric and oligomeric forms of actin, and not to monomeric actin. Fluorescent conjugates of phalloidin are commonly used to label actin filaments for immunocytochemical and immunohistochemical applications. Both rhodamine and fluorescein labeled phalloidin can be used to localize and quantify actin filaments in cells and tissues.

Short Description

Phalloidin conjugated to FITC via the side chain of amino acid 7.

Reactivity

Chicken, Fish, Human, Mouse, and Rat

Conjugation

FITC Ex: 495 nm, Em: 516 nm

Applications

ICC, IHC

Buffer

Phosphate-buffered saline, 50% glycerol, 1 mg/ml BSA, and 0.05% sodium azide.

Molecular Weight

1250 kDa

Shipping Conditions

Blue Ice

Storage Conditions

Store at –20°C. Stable for 1 year.

IHC Dilution

1:500

Specificity

Phalloidin: FITC can be used for immunocytochemical and immunohistochemical labeling of actin filaments. Phalloidin: FITC binds polymeric and oligomeric actin, but does not bind monomeric actin. The conjugates can be used to label actin filaments in many species, including human, mouse, rat, chicken, and fish.