TNF-Alpha, Soluble (human) (rec.)

Source : E. coli Binds to human and mouse TNF-R1.Tumor necrosis factor (TNF, cachexin or cachectin and formerly known as tumor necrosis factor-Alpha) is a cytokine involved in systemic inflammation and is a member of a group of cytokines that stimulate the acute phase reaction. The receptor TNF-R1 is activated by both the membrane-bound and soluble trimeric forms of TNF-Alpha, whereas the receptor TNF-R2 only responds to the membrane-bound form of TNF-Alpha (MultimericTNF-Alpha) . Since the MultimericTNF-Alpha mimics the membrane-bound form (forms oligomers higher than trimer), it is the only TNF-Alpha protein that can activate the TNF-R2. For TNF-R1 activation, either normal" TNF-Alpha or MultimericTNF-Alpha can be used."

Product Specifications

Product Name Alternative

Tumor Necrosis Factor Ligand Superfamily Member 2; Cachectin

Reactivity

Mouse, Human

Purification

>=95% (SDS-PAGE)

Components

Reconstitution 10 μg size: Reconstitute with 100 μl sterile water. 50 μg size: Reconstitute with 50 μl sterile water. PBS containing at least 0.1% BSA should be used for further dilutions. Concentration 10 μg size: 0.1 mg/mL after reconstitution. 50 μg size: 1 mg/mL after reconstitution. Formulation: Lyophilized. Contains PBS.

Storage Conditions

Stable for at least 6 months after receipt when stored at -20°C. Working aliquots are stable for up to 3 months when stored at -20°C.

Applications Notes

MW :~17kDa (SDS-PAGE) ; Biological Activity:Induces cell death at a concentration range of 0.01-10ng/ml (ED50= 0.05ng/ml or 2 x 10E7 Units/mg; WEHI 164 cells) . Endotoxin Content <0.01EU/μg purified protein (LAL test; Lonza) .