RSPO1 Mouse

Source: Sf9, Baculovirus cells.Sterile Filtered colorless solution.R-Spondin-1 (Rspo1) is a part of the Rspondin family. Rspo1 plays a role as an activator of the canonical Wnt signaling pathway by acting as a ligand for LGR4-6 receptors. Rspo1 induces the onset of crypt cell proliferation and increases intestinal epithelial healing effect. Rspo1 is negatively regulating the TGF-beta pathway.RSPO1 produced in Sf9 Baculovirus cells is a single, glycosylated polypeptide chain containing 254 amino acids (21-265a.a.) and having a molecular mass of 28.2kDa (Molecular size on SDS-PAGE will appear at approximately 28-40kDa) . RSPO1 is expressed with an 9 amino acid His tag at C-Terminus and purified by proprietary chromatographic techniques.

Product Specifications

Product Name Alternative

R-spondin-1, Cristin-3, mCristin-3, Roof plate-specific spondin-1, Rspo1, RSPO, R-spondin 1, R-spondin, Rspondin, CRISTIN3.

Purification

Greater than 90.0% as determined by SDS-PAGE.

Components

RSPO1 protein solution (0.5mg/mL) contains Phosphate Buffered Saline (pH 7.4) and 10% glycerol.

Storage Conditions

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) . Avoid multiple freeze-thaw cycles.

Amino Acids

ADPSRGIKGK RQRRISAEGS QACAKGCELC SEVNGCLKCS PKLFILLERN DIRQVGVCLP SCPPGYFDAR NPDMNKCIKC KIEHCEACFS HNFCTKCQEG LYLHKGRCYP ACPEGSTAAN STMECGSPAQ CEMSEWSPWG PCSKKRKLCG FRKGSEERTR RVLHAPGGDH TTCSDTKETR KCTVRRTPCP EGQKRRKGGQ GRRENANRHP ARKNSKEPGS NSRRHKGQQQ PQPGTTGPLT SVGPTWAQHH HHHH.