Human Bone Morphogenetic Protein-2 (AF)

Source: Genetically modified E.coli. Predicted MW: Dimer, 13.0/26.1 kDa (115/230 aa) Bone morphogenetic protein 2 (BMP-2) is a member of the bone morphogenetic protein (BMP) family and functions as a potent inducer of bone and cartilage development. BMP proteins are synthesized as large precursor molecules which are cleaved by proteolytic enzymes. Active BMP-2 consists of forming a homodimer or a heterodimer with a related BMP, such as BMP-7. BMP-2 signals through type I and type II receptor tyrosine kinases in conjuction with SMAD proteins to directly promote osteoblast differentiation. BMP-2 is also important during cardiac development and supports epicardial cell migration.

Product Specifications

Product Name Alternative

BMP-2A, BMP2A

Gene Name

BMP2

Gene ID

650

UniProt

P12643

Purification

Reducing and Non-Reducing SDS PAGE at >= 95%

Components

Lyophilized from a sterile (0.2 micron) filtered aqueous solution containing 0.1% Trifluoroacetic Acid (TFA) Sterile water at 0.1 mg/mL

Shipping Conditions

Room temperature

Storage Conditions

Store at -20°C

Applications Notes

Endotoxin: Less than 0.1 ng/μg (1 IEU/μg) as determined by LAL test. Biological Activity was determined by Alkaline phosphatase activity induced in ATDC-5 cells at <= 250 ng/mL; >= 4.0 x 10^3 units/mg .Centrifuge vial before opening, Suspend the product by gently pipetting the above recommended solution down the sides of the vial. DO NOT VORTEX. Allow several minutes for complete reconstitution. For prolonged storage, dilute to working aliquots in a 0.1% BSA solution, store at -80°C and avoid repeat freeze thaws. Upon reconstitution, a small amount of visible precipitate can be expected. A 10% overfill has been added to the total material vialed to compensate for this loss.