EPOA Mouse

Source: Sf9, Baculovirus cells.Sterile Filtered colorless solution.Erythropoietin or EPO is a hormone (glycoprotein), part of the type I cytokine group of proteins. EPO is found mainly in the kidney tissue, produced from fibroblast-like cortical interstitial cells near the proximal tubules. EPO is also present in the blood, where it acts as red cell production regulator, by the promotion of differentiation of erythroid and thereby starts hemoglobin synthesis. Furthermore, EPO has neuroprotective activity towards brain injuries & anti-apoptotic activity in different tissues.EPOA Mouse Recombinant produced in Baculovirus is a single glycosylated polypeptide chain containing 176 amino acids (27-192 aa) and having a molecular mass of 19.8kDa.EPOA is fused to a 9 amino acid His tag at C-terminus and purified by proprietary chromatographic techniques.

Product Specifications

Product Name Alternative

Erythropoietin, erythropoietin isoform 1 precursor, Epo.

Applications

Functional Assay

Purification

Greater than 95.0% as determined by SDS-PAGE.

Components

EPOA protein (0.25mg/mL) contains 10% glycerol and Phosphate-Buffered Saline (pH 7.4) .

Storage Conditions

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) .Avoid multiple freeze-thaw cycles.

Applications Notes

Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 range

Amino Acids

ADPMAPPRLI CDSRVLERYI LEAKEAENVT MGCAEGPRLS ENITVPDTKV NFYAWKRMEV EEQAIEVWQG LSLLSEAILQ AQALLANSSQ PPETLQLHID KAISGLRSLT SLLRVLGAQK ELMSPPDTTP PAPLRTLTVD TFCKLFRVYA NFLRGKLKLY TGEVCRRGDR HHHHHH.