Recombinant E.Coli Heat Shock Protein Cofactor 70 (HSP24)

Source : Escherichia Coli. GrpE Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 197 amino acids and having a molecular mass of 21.8 kDa. The GrpE is purified by proprietary chromatographic techniques. GrpE , co-chaperone of E.coli, participates actively in the response to hyperosomotic and heat shock by preventing the aggregation of stress-denatured proteins in association with DnaK. This protein is the nucleotide exchange factor for DnaK and may function as a thermosensor. Several rounds of ATP-dependent interactions between DnaJ, DnaK and GrpE are required for fully efficient folding.

Product Specifications

Product Name Alternative

HSP24||HSPB25.3||HSP-70 Cofactor||Protein grpE||Heat shock protein B25.3||grpE||b2614||JW2594.

Purification

Greater than 90.0% as determined by analysis by SDS-PAGE.

Components

The HSP24 protein solution contains 20mM Tris-HCl pH-8 and 0.1M NaCl.

Storage Conditions

GrpE although stable 4°C for 4 weeks, should be stored desiccated below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) .Please prevent freeze-thaw cycles.

Amino Acids

MSSKEQKTPE GQAPEEIIMD QHEEIEAVEP EASAEQVDPR DEKIANLEAQ LAEAQTRERD GILRVKAEME NLRRRTELDI EKAHKFALEK FINELLPVID SLDRALEVAD KANPDMSAMV EGIELTLKSM LDVVRKFGVE VIAETNVPLD PNVHQAIAMV ESDDVAPGNV LGIMQKGYTL NGRTIRAAMV TVAKAKA.