Recombinant Human Wntless

Source : E.coli. WLS Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 155 amino acids (101-232a.a) and having a molecular mass of 17.8kDa. WLS is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. WLS (Wnt trafficking regulator) is a multipass transmembrane protein which regulates the sorting and secretion of Wnt protein. WLS is necessitated for organogenesis during embryogenesis and for the establishment of the anterior-posterior axis. WLS is encoded by a gene which maps to human chromosome 1 and is expressed as multiple alternatively spliced isoforms. WLS promotes various cancers such as glioma tumourigenesis.

Product Specifications

Product Name Alternative

C1orf139||EVI||GPR177||MRP||Protein wntless homolog||Integral membrane protein GPR177||Protein evenness interrupted homolog||Putative NF-kappa-B-activating protein 373||WLS.

Purification

Greater than 90% as determined by SDS-PAGE.

Components

WLS protein solution (1mg/mL) containing 20mM Tris-HCl buffer (pH 8.0),0.4M Urea and 10% glycerol.

Storage Conditions

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) .Avoid multiple freeze-thaw cycles.

Amino Acids

MGSSHHHHHH SSGLVPRGSH MGSMEMSPWF QFMLFILQLD IAFKLNNQIR ENAEVSMDVS LAYRDDAFAE WTEMAHERVP RKLKCTFTSP KTPEHEGRYY ECDVLPFMEI GSVAHKFYLL NIRLPVNEKK KINVGIGEIK DIRLVGIHQN GGFTK.