Recombinant Human Regucalcin

Source : Escherichia Coli. RGN Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 319 amino acids (1-299 a.a.) and having a molecular mass of 35.4kDa.RGN is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. Regucalcin (RGN) is a member of the SMP-30/CGR1 family. Regucalcin is a Ca (2+) -binding protein which does not contain EF-hand motif of Ca (2+) -binding domain. RGN has a critical role in the keep of intracellular Ca2+ homeostasis due to activating of Ca2+ pump enzymes in the plasma membrane (basolateral membrane), microsomes (endoplasmic reticulum) and mitochondria of many cells. Moreover, RGN plays a multifunctional role in the regulation of cell functions in the liver, kidney cortex, heart and brain and a suppressor protein for cell signaling systems in many cell types.

Product Specifications

Product Name Alternative

Regucalcin||RC||Gluconolactonase||GNL||Senescence marker protein 30||SMP-30||RGN||SMP30.

Purification

Greater than 85.0% as determined by SDS-PAGE.

Components

RGN protein solution (0.5mg/mL) containing 20mM Tris-HCl, pH8.0, 2M Urea and 20% Glycerol.

Storage Conditions

Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) .Avoid multiple freeze-thaw cycles.

Amino Acids

MGSSHHHHHH SSGLVPRGSH MSSIKIECVL PENCRCGESP VWEEVSNSLL FVDIPAKKVC RWDSFTKQVQ RVTMDAPVSS VALRQSGGYV ATIGTKFCAL NWKEQSAVVL ATVDNDKKNN RFNDGKVDPA GRYFAGTMAE ETAPAVLERH QGALYSLFPD HHVKKYFDQV DISNGLDWSL DHKIFYYIDS LSYSVDAFDY DLQTGQISNR RSVYKLEKEE QIPDGMCIDA EGKLWVACYN GGRVIRLDPV TGKRLQTVKL PVDKTTSCCF GGKNYSEMYV TCARDGMDPE GLLRQPEAGG IFKITGLGVK GIAPYSYAG.