DUSP23 Recombinant Protein

Source : Escherichia Coli. DUSP23 Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 170 amino acids (1-150 a.a.) and having a molecular mass of 18.8kDa.DUSP23 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. DUSP23 is a member of the protein-tyrosine phosphatase family. DUSP23 is a protein phosphatase which facilitates dephosphorylation of phosphorylated proteins on Tyr and Ser/Thr residues. In vitro, DUSP23 dephosphorylate p44-ERK1 (MAPK3) but not p54 SAPK-beta (MAPK10) . In addition, DUSP23 enhances activation of JNK and p38 (MAPK14) .

Product Specifications

Product Name Alternative

Dual specificity protein phosphatase 23||DUSP25||Low molecular mass dual specificity phosphatase 3||LDP-3||VHZ||VH1-like phosphatase Z||MOSP||RP11-190A12.1||FLJ20442||EC 3.1.3.16||EC 3.1.3.48.

Purification

Greater than 90.0% as determined by SDS-PAGE analysis.

Components

DUSP23 protein solution (1mg/mL) containing 20mM Tris-HCl buffer (pH8.0), 100mM NaCl, 2mM DTT, and 10% glycerol.

Storage Conditions

Store at 4°C if entire vial will be used within 2-4 weeks.Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) .Avoid multiple freeze-thaw cycles.

Amino Acids

MGSSHHHHHH SSGLVPRGSH MGVQPPNFSW VLPGRLAGLA LPRLPAHYQF LLDLGVRHLV SLTERGPPHS DSCPGLTLHR LRIPDFCPPA PDQIDRFVQI VDEANARGEA VGVHCALGFG RTGTMLACYL VKERGLAAGD AIAEIRRLRP GSIETYEQEK AVFQFYQRTK