RM CSF Recombinant Protein

Source : HEK293 MCSF Rat Recombinant produced in HEK-293 cells is a secreted protein (amino acids Glu33-Arg254) . M-CSF is disulfide-linked homodimer containing 2 x 222 a.a chains. Granulocyte/Macrophage Colony-Stimulating Factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. CSF-1 induces cells of the monocyte/macrophage lineage. It plays a role in immunological defenses, bone metabolism, lipoproteins clearance, fertility and pregnancy.

Product Specifications

Product Name Alternative

CSF-1||Lanimostim||MCSF||MGC31930||M-CSF.

Purification

Greater than 95.0% as determined by SDS-PAGE.

Components

The recombinant MCSF was lyophilized after extensive dialysis against PBS.

Storage Conditions

Lyophilized MCSF although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution MCSF should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) .Please prevent freeze-thaw cycles.

Applications Notes

It is recommended to reconstitute the MCSF in sterile PBS not less than 100 μg/ml, which can then be further diluted to other aqueous solutions. The ED50 of 3-4ng/ml is measured by its ability to stimulate the proliferation of mouse M-NFS-60 cells.