TNFSF13B Protein (N-His & AVI, Human)
Product Specifications
Abbreviation
TNFSF13B
Synonyms
BAFF, BLYS, CD257, DTL, TALL-1, TALL1, THANK, TNFSF20, TNLG7A, UNQ401/PRO738, ZTNF4
Host
HEK 293
Tag
N-His & AVI
Endotoxin
< 1.0 EU per μg protein as determined by the LAL method.
Purity
≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-MALS.
Bioactivity
1.Immobilized Recombinant Human BAFFR / TNFRSF13C / CD268 Protein (Fc Tag) (Cat: 16079-H02H) at 2 μg/mL (100 μL/well) can bind Recombinant Human BAFF/BLyS/TNFSF13B Protein (His & AVI Tag), Biotinylated (Cat: 10056-H56H1-B), the EC50 is 2-6 ng/mL (QC tested) . 2.Immobilized Recombinant Human TNFRSF17 / BCMA (CD269) Protein (ECD, Fc tag) (Cat: 10620-H02H) at 2 μg/mL (100 μL/well) can bind Recombinant Human BAFF/BLyS/TNFSF13B Protein (His & AVI Tag), Biotinylated (Cat: 10056-H56H1-B), the EC50 is 0.5-1.5 ng/mL (Routinely tested) . 3.Immobilized Recombinant Human TACI / TNFRSF13B Protein (ECD, Fc Tag) (Cat: 29965-H02H) at 2 μg/mL (100 μL/well) can bind Recombinant Human BAFF/BLyS/TNFSF13B Protein (His & AVI Tag), Biotinylated (Cat: 10056-H56H1-B), the EC50 is 0.8-3 ng/mL (Routinely tested) . 4.Loaded Biotinylated Recombinant Human BAFF/BLyS/TNFSF13B Protein (His & AVI Tag) (Cat. No. 10056-H56H1-B) on SA Biosensor, can bind Recombinant Human BCMA Protein (ECD, His Tag) (Cat. No. 10620-H08H) with an affinity constant of 0.853 μM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) .
Function
Ala134-Leu285
Molecular Weight
The recombinant Human TNFSF13B comprises 219 amino acids and has a predicted molecular mass of 24.24 kDa. It migrates as approximately 56.4 kDa and 30.2 band in SDS-PAGE under reducing conditions. The molecular weight of this protein is around 77.2 kDa verified by SEC-MALS.
Species
Human
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