MMP2 Protein (Native, Human)
Product Specifications
Abbreviation
MMP2
Synonyms
CLG4, CLG4A, MMP-2, MMP-II, MONA, TBE-1
Host
HEK 293
Tag
Native
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method
Purity
≥ 90 % as determined by SDS-PAGE ≥ 90 % as determined by SEC-HPLC.
Bioactivity
1. Measured by its ability to cleave the fluorogenic peptide substrate Mca-PLGL-Dpa-AR-NH2 (AnaSpec, Catalog # 27076) . The specific activity is > 1,000 pmoles/min/μg. (Activation description: The proenzyme needs to be activated by APMA for an activated form) 2. Measured by its binding ability in a functional ELISA. Immobilized Human MMP-2 (Cat:10082-HNAH) at 2 μg/ml (100 μl/well) can bind Human TIMP2 hFc (Cat:10396-H01H), the EC50 of Human TIMP2 hFc is 6.0-30.0 ng/mL.
Function
Met1-Cys660
Molecular Weight
The recombinant human MMP2 consists of 631 amino acids and migrates as an 72 kDa band as predicted in SDS-PAGE under reducing conditions.
Species
Human
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