FLT1 Protein (C-His-AVI, Human)

Product Specifications

Abbreviation

FLT1

Synonyms

FLT, FLT-1, FRT, VEGFR-1, VEGFR1

Host

HEK 293

Tag

C-His-AVI

Endotoxin

< 1.0 EU per μg protein as determined by the LAL method.

Purity

≥ 95 % as determined by SDS-PAGE. ≥ 95 % as determined by SEC-HPLC.

Bioactivity

1.Loaded Biotinylated Recombinant Human VEGFR1/FLT1 Protein, His & AVI Tag (Cat. No. 10136-H49H-B) on SA Biosensor, can bind Recombinant VEGF165 Protein, Human, Cynomolgus (Cat. No. 11066-HNAH) with an affinity constant of 0.506 nM as determined in BLI assay (Sartorius Octet RED384) (Routinely tested) . 2.Immobilized Recombinant Human VEGFR1 / FLT-1 Protein (ECD, His & AVI Tag), Biotinylated (Cat: 10136-H49H-B) at 2 μg/mL (100 μL/well) on streptavidin precoated (2 μg/mL, 100 μL/well) can bind Recombinant Human / Cynomolgus VEGF / VEGFA / VEGF165 Protein (Cat: 11066-HNAH), the EC50 is 58-174 ng/mL (Routinely tested) . 3.Immobilized Recombinant Human / Cynomolgus VEGF / VEGFA / VEGF165 Protein (Cat: 11066-HNAH) at 2 μg/mL (100 μL/well) can bind Recombinant Human VEGFR1 / FLT-1 Protein (ECD, His & AVI Tag), Biotinylated (Cat: 10136-H49H-B), the EC50 is 8-30 ng/mL (QC tested) .

Function

Met1-Asn756

Molecular Weight

The recombinant Human FLT1 consists of 756 amino acids and predicts a molecular mass of 85.45 kDa. It migrates as an approximately 113.32 kDa band in SDS-PAGE under reducing conditions.